Nunclon Surface Cell Line Data

We’ve tested the most commonly used cell lines on Thermo Scientific Nunclon Delta and Nunclon Sphera cell culture plastics side-by-side with the leading competitor to validate Nunc cell culture plastics’ place in your cell culture hood.

Look up your cell line and see for yourself how they thrive in Nunc cell culture plastics. We are adding data for more cells all the time.

A431 cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in: 

• Morphology
• Cell viability and growth
• Gene expression
   

The images above are representative brightfield images of A431 cells grown on Nunclon Delta (left) and Corning® tissue culture (TC)–treated surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
 

(A) Mean cell viability of A431 cells grown on Nunclon Delta or Corning® TC surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of A431 cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
 

For the above Nunclon Delta surface vs Corning TC Surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.  

A549 cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in: 

• Morphology
• Cell viability and growth
• EGFR activation

The images above are representative brightfield images of A549 cells grown on Nunclon Delta (left) and Corning® tissue culture (TC)–treated surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
 

(A) Mean cell viability of A549 cells grown on Nunclon Delta or Corning® TC surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of A549 cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

A549 cells grown on Nunclon Delta (Nunc) or Corning® TC (Corning) surfaces as well as those switched to Nunclon Delta (S to ND) surface, were treated with epidermal growth factor (EGF, 200 ng/mL) for 10 minutes. Total cell lysates (30 mg) were subjected to western blot analysis for EGFR phosphorylation using anti-phospho-EGFR (Tyr1086) Rabbit Polyclonal Antibody 0.5 µg/mL. Total EGFR and GAPDH were used as experimental and normalization controls respectively.
 

For the above Nunclon Delta surface vs Corning® TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco IDMD, 10% Gibco FBS, and 1% Penicillin Streptomycin.

Caco-2 cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in: 

• Morphology
• Cell viability and growth
• Cell attachment

The images above are representative brightfield images of Caco-2 cells grown on Nunclon Delta (left) and Corning® tissue culture (TC)–treated surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
 

(A) Mean cell viability of Caco-2 cells grown on Nunclon Delta or Corning® TC surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of Caco-2 cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

As an extension of cell attachment and growth, Caco-2 cells grown on Nunclon Delta (left) and Corning® TC surfaces (middle), and those switched to Nunclon Delta (ND) surface (right) were stained for the tight junction protein ZO-3, shown in green. The nucleus was stained with DAPI (shown in blue). No morphological difference was observed between the surfaces. Cells were grown on 6-well plates and stained on the same surface. Images were captured using EVOS M5000 imaging system under 20X magnification and cropped using the same aspect ratio for better visualization.

For the above Nunclon Delta surface vs Corning® TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 20% Gibco FBS, and 0.5% Penicillin Streptomycin.

CHO-K1 cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in: 

• Morphology
• Cell viability and growth
• Transfection efficiency

The images above are representative brightfield images of CHO-K1 cells grown on Nunclon Delta (left) and Corning® tissue culture (TC)–treated surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.

(A) Mean cell viability of CHO-K1 cells grown on Nunclon Delta or Corning® TC surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of CHO-K1 cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

Flow cytometry evaluations of transfection efficiency of mCherry in CHO-K1 cells. 10,000 events were captured per sample. n=2. Error bars represent SEM. UT - Untransfected cells, T – Transfected cells.

For the above Nunclon Delta surface vs Corning® TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.  

Cos-7 cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in: 

• Morphology
• Cell viability and growth

The images above are representative brightfield images of Cos-7 cells grown on Nunclon Delta (left) and Corning® tissue culture (TC)–treated surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
 

(A) Mean cell viability of Cos-7 cells grown on Nunclon Delta or Corning® TC surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of Cos-7 cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

For the above Nunclon Delta surface vs Corning® TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.  

HEK293 cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in:

• Morphology
• Cell viability and growth
• Transfection efficiency
   

The images above are representative brightfield images of HEK293 cells grown on Nunclon Delta (left) and Corning® surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems 10X magnification.

(A) Mean cell viability of HEK293 cells grown on Nunclon Delta or Corning® TC surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of HEK293 cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
 

HEK293 cells seeded on Nunclon Delta or Corning® TC surfaces and transfected with mCherry using Lipofectamine 3000 transfection reagent. Images of live cells were acquired 24 hours post transfection.

Flow cytometry evaluations of transfection efficiency of mCherry in HEK293 cells (as shown above). 10,000 events were captured per sample. n = 2. Error bars represent SEM. UT – Untransfected cells, T – Transfected cells.

For the above Nunclon Delta surface vs Corning® TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.  

HeLa cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in: 

• Morphology
• Cell viability and growth
• Transfection efficiency
   

The images above are representative brightfield images of HeLa cells grown on Nunclon Delta (left) and Corning® tissue culture (TC)–treated surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
 

(A) Mean cell viability of HeLa cells grown on Nunclon Delta or Corning® TC surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of HeLa cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
 

HeLa cells seeded on Nunclon Delta or Corning® TC surfaces (6-well plates) and transfected with mCherry using Lipofectamine 3000 transfection reagent Images of live cells were acquired 24 hours post transfection.

Flow cytometry evaluations of transfection efficiency of mCherry in HeLa cells (as shown above). 10,000 events were captured per sample. n = 2. Error bars represent SEM. UT – Untransfected cells, T – Transfected cells.

For the above Nunclon Delta surface vs Corning® TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.  

HepG2 cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in: 

• Morphology
• Cell viability and growth
• Gene expression
• See 3D cell culture data for cell cultures grown on Nunclon Sphera vs the leading competitor
   

The images above are representative brightfield images of HepG2 cells grown on Nunclon Delta (left) and Corning® tissue culture (TC)–treated surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
 

(A) Mean cell viability of HepG2 cells grown on Nunclon Delta or Corning® tissue culture (TC) treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of HepG2 cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
 

For the above Nunclon Delta surface vs Corning® TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.  

HT-29 cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in:

• Morphology
• Cell viability and growth
• Protein expression
   

The images above are representative brightfield images of Ht29 cells grown on Nunclon Delta (left) and Corning® tissue culture (TC) treated surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
 

(A) Mean cell viability of HT-29 cells grown on Nunclon Delta or Corning® tissue culture (TC) treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of HT-29 cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
 

(A) HT-29 cells grown on Nunclon Delta (left panel) and Corning® TC surfaces (right panel) 6-well plates were fixed on the plate and stained for the junction protein ZO-3 using ABfinity Anti-ZO-3 Recombinant Rabbit Oligoclonal Antibody (5 mg/mL) and detected using goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate (1:2,000). Panel a shows representative cells that were stained for detection and localization of ZO-3 protein (green), Panel b represents cytoskeletal F-actin staining using Alexa Fluor 555 Rhodamine Phalloidin (1:300). Panel c is stained for nuclei (blue) using Hoechst 33342 (1:2,000). Panel d is a composite image of Panels a, b and c demonstrating membrane localization of ZO-3. Scale bar=100 μm. (B) As an extension of cell growth and confluency, ZO-3 expression was further detected by western blot at days 2 and 6 of HT-29 cells grown on Nunclon Delta (Nunc) or Corning® TC surfaces (Corning) within 100 mm dishes, as well as those switched to Nunclon Delta surface (S to ND). The blot was probed with 1 µg/mL of ZO-3 antibody. A 140 kDa band corresponding to ZO-3 was observed across all lanes. Additionally, a 100 kDa band corresponding to ZO-3 isoform was specifically expressed in confluent cells (6 days).

For the above Nunclon Delta surface vs Corning® TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco IMDM, 10% Gibco FBS, and 1% Penicillin Streptomycin.

HepG2 cells grown on Nunc Nunclon Sphera surface, in combination with Gibco media and Gibco FBS have been proven to yield consistent, quality spheroids.

In a side-by-side comparison with a leading supplier, Nunclon Sphera yielded high-quality HepG2 spheroids, while the other supplier’s product yielded undesirable satellite colonies.

Spheroid formation in Nunclon Sphera and Corning® ULA U-bottomed surface: HepG2 cells were seeded at the respective densities on Nunclon Sphera and Corning® ULA™ U-bottomed plates for spheroid generation. On Day 6, brightfield images of spheroids were captured with EVOS  imaging system using 4X objective. The figure shows representative images. Spheroids formed on the Sphera surface had no satellite colonies, while those grown on Corning® ULA™ plates were found to have satellite colonies  a higher seeding densities. Scale bar=1,000 μm.

See the protocol for HepG2 spheroid generation

LNCaP cells grown on Nunc Nunclon Sphera surface, in combination with Gibco media and Gibco FBS have been proven to yield consistent, quality spheroids.

In a side-by-side comparison with a leading supplier, Nunclon Sphera yielded high-quality LNCaP spheroids, while the other supplier’s product yielded undesirable satellite colonies.

Spheroid formation in Nunclon Sphera and Corning® ULA U-bottomed surface. LNCaP cells were seeded at the respective densities on Nunclon Sphera and Corning® ULA U-bottomed plates for spheroid generation. On day 4, brightfield images of spheroids were captured with EVOS  imaging system using 4X objective. The figure shows representative images. Spheroids formed on the Sphera surface had no satellite colonies, while those grown on Corning® ULA plates were found to have satellite colonies at higher seeding densities. Scale bar=650 μm

See the protocol for LNCaP spheroid generation

MCF-7 cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in:

• Morphology
• Cell viability and growth
• Surface marker expression
   

The images above are representative brightfield images of MCF-7 cells grown on Nunclon Delta (left) and Corning® tissue culture (TC) treated surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
 

(A) Mean cell viability of MCF-7 cells grown on Nunclon Delta or Corning® tissue culture (TC) treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of MCF-7 cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
 

MCF-7 cells grown on Nunclon Delta (left) or Corning TC surfaces (middle) as well as those switched to Nunclon Delta surface (right) were analyzed for the expression of endogenous receptor tyrosine protein kinase ErbB2 using flow cytometry. IgG1 was used as the isotype control. No difference was observed in the relative expression of ErbB2 using the different growth surfaces. Samples were run in duplicate on an Invitrogen Attune NxT Flow Cytometer.

For the above Nunclon Delta surface vs Corning® TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco MEM, 10% Gibco FBS, 1% Penicillin Streptomycin, and 0.01mg/ml Insulin.

MCF-10A cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in:

• Morphology
• Cell viability and growth
• Gene expression
   

The images above are representative brightfield images of MCF-10A cells grown on Nunclon Delta (left) and Corning® tissue culture (TC) treated surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.

(A) Mean cell viability of MCF-10A cells grown on Nunclon Delta or Corning® tissue culture (TC) treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of MCF-10A cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).

For the above Nunclon Delta surface vs Corning® TC surface experiments, the following formulations of cell culture media and reagents were used: Reconstituted Gibco Medium 171, 100 ng/ml cholera toxin, and 1% Penicillin Streptomycin.  

MDA-MB-231 cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in:

• Morphology
• Cell viability and growth
• Surface marker expression
   

The images above are representative brightfield images of MDA-MB-231 cells grown on Nunclon Delta (left) and Corning® tissue culture (TC) treated surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
 

(A) Mean cell viability of MDA-MB-231 cells grown on Nunclon Delta or Corning® tissue culture (TC) treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of MDA-MB-231 cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
 

MDA-MB-231 cells grown on Nunclon Delta or Corning® TC surface for 25 doublings as well as those switched to Nunclon Delta (ND) surface for 10 doublings were assessed for the expression of two endogenous surface marker proteins: ICAM-1 (top row, 0.25 μg/test) and CD44 (bottom row, 0.06 μg/test) using flow cytometry. 10,000 events were collected for each condition. No difference was observed in the surface marker expression between different growth surfaces.

For the above Nunclon Delta surface vs Corning® TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.  

MDCK cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in:

• Morphology
• Cell viability and growth
• Relative gene expression
   

The images above are representative brightfield images of MDCK cells grown on Nunclon Delta (left) and Corning® tissue culture (TC) treated surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
 

(A) Mean cell viability of MDCK cells grown on Nunclon Delta or Corning® tissue culture (TC) treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of MDCK cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
 

For the above Nunclon Delta surface vs Corning® TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.  

NIH3T3 cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in:

• Morphology
• Cell viability and growth
• Wound-healing assay
   

The images above are representative brightfield images of NIH3T3 cells grown on Nunclon Delta (left) and Corning® tissue culture (TC) treated surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
 

(A) Mean cell viability of NIH3T3 cells grown on Nunclon Delta or Corning® tissue culture (TC) treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of NIH3T3 cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
 

Wound-healing assay. (A) NIH3T3 cells grown on Nunclon Delta or Corning TC surfaces were assessed for cell health by their ability to undergo wound healing under serum-starved conditions on the respective surfaces. (B) Quantification of images shown in A. Images were analyzed using ImageJ. The percent of wounded area over time is plotted on the y-axis. Error bars represent SEM. Data are collected from 3 separate fields for each condition. Images were captured using EVOS Cell Imaging Systems under 10X magnification.

For the above Nunclon Delta surface vs Corning® TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.  

PC-3 cells grown on Nunc Nunclon Delta surface, in combination with Gibco media and Gibco FBS have been tested to support consistent cell growth.

Cell cultures tested side by side with a leading competitor for proven comparable results in:

• Morphology
• Cell viability and growth
• Transfection efficiency
   

The images above are representative brightfield images of PC-3 cells grown on Nunclon Delta (left) and Corning® tissue culture (TC) treated surfaces (middle), and those switched to Nunclon Delta (ND) surface for 10 passage doublings (right). Images were captured using EVOS Cell Imaging Systems under 10X magnification.
 

(A) Mean cell viability of PC-3 cells grown on Nunclon Delta or Corning® tissue culture (TC) treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. (B) Mean population doubling time of PC-3 cells propagated on Nunclon Delta and Corning TC treated surfaces (for 30 population doublings), and of those switched to Nunclon Delta TC treated surface (for 10 passage doublings) is plotted on the y-axis. Error bars represent SEM. n.s. = not significant (by Student’s unpaired t-test).
 

PC-3 cells seeded on Nunclon Delta or Corning® tissue culture (TC) treated surfaces and transfected with mCherry using Lipofectamine 3000 transfection reagent. Images of live cells were acquired 24 hours post transfection.

Flow cytometry evaluations of transfection efficiency of mCherry in PC-3 cells (as shown above). 10,000 events were captured per sample. n = 2. Error bars represent SEM. UT – Untransfected cells, T – Transfected cells.

For the above Nunclon Delta surface vs Corning® TC surface experiments, the following formulations of cell culture media and reagents were used: Gibco DMEM, 10% Gibco FBS, and 1% Penicillin Streptomycin.  

T47D cells grown on Nunc Nunclon Sphera surface, in combination with Gibco media and Gibco FBS have been proven to yield consistent, quality spheroids.

In a side-by-side comparison with a leading supplier, Nunclon Sphera yielded high-quality T47D spheroids, while the other supplier’s product yielded undesirable satellite colonies.

Spheroid formation in Nunclon Sphera and Corning® ULA™ U-bottomed surface: T47D cells were seeded at the respective densities on Nunclon Sphera and Corning® ULA™ U-bottomed plates for spheroid generation. On Day 6, brightfield images of spheroids were captured with EVOS M5000 imaging system using 4X objective. The figure shows representative images. Spheroids formed on the Sphera surface had no satellite colonies, while those grown on Corning® ULA™ plates were found to have satellite colonies for cell seeding numbers 2,000 and above, ~75% of the time. Experiments were repeated at least 3 times. Scale bar=800 μm.

See the protocol for T47D spheroid generation
See more 3D cell culture comparisons for other cell lines